Bulletin of ANPA

Abstract submitted to ANPA Conference July 14–16, 2023

Volume 5, Number 1

Biological, Medical, Soft Matter and Chemical Physics

Abstract ID: ANPA2023-N00039

Abstract:

ANPA2023-N00039: Direct Visualization of Antimicrobial Peptide - Induced Defects on Supported Lipid Bilayer

Authors:

• Olivia Walsh; California State Polytechnic University, Pomona

• Leona Choi; California State Polytechnic University, Pomona

• Krishna Sigdel;

Antimicrobial peptides (AMPs) play a crucial role in the innate immune systems of various organisms. AMPs interact with bacterial and mammalian cell membranes through various mechanisms, varying from nanopore formation to microscale membrane lysis process. The interactions between AMPs and cell membranes have been studied extensively through various biochemical assays; they are thought to kill pathogens through cell membrane intervention. However, the mechanistic details behind membrane destabilization are still elusive. In this study, we used atomic force microscopy (AFM) to explore the effects of an AMP CM15 on model E. coli lipid membranes. CM15 is a hybrid peptide composed of Cecropin-A and melittin from bee venom. Our study sheds light on how CM15 interacts with a supported lipid bilayer.

To cite this abstract, use the following reference: https://anpaglobal.org/conference/2023/ANPA2023-N00039