Bulletin of ANPA

Abstract submitted to ANPA Conference July 14–16, 2023

Volume 5, Number 1

Biological, Medical, Soft Matter and Chemical Physics

Abstract ID: ANPA2023-N00035

Abstract:

ANPA2023-N00035: Nanoconfined based Single-Entity Analysis: Unveiling Small Molecule Detection and Intermolecular Interactions in crowded environments.

Authors:

• Santosh Khatri; Florida International University

• Jin He; Florida International University

The behavior of individual molecules plays a crucial role in biochemical processes, which in turn influence the properties of cells and ultimately impact human behavior. Nanopore sensing, a cutting-edge technique for detecting and studying molecules at the single-molecule level, holds immense promise in unraveling the intricate details of molecular behavior. However, the sensitivity of existing nanopore methods, such as nanopipettes, is limited by the small size and fast speed of biomolecules. To address this problem, a novel approach of nanoconfinement at the nanopipette tip has been developed. This innovative method allows for the detection of smaller biomolecules, such as DNA bases and short peptides, using a nanopipette with a pore size over 10 times larger than the size of the biomolecule. The technique involves backfilling the nanopipette with biomolecules and then driving them out using concentration gradient and/or nanopore bias. This breakthrough enables deeper insights into the intermolecular interactions of various biomolecules and enables the detection of small single molecules at a scale of nearly 1 nanometer. In summary, the ability to detect and study small biomolecules with enhanced sensitivity using nanopipettes opens an exciting opportunity for understanding the intricate world of molecular behavior and has the potential to greatly impact fields such as drug discovery, personalized medicine, and biotechnology, opening new avenues for research and application in the field of molecular sciences.

To cite this abstract, use the following reference: https://anpaglobal.org/conference/2023/ANPA2023-N00035